c)Bacterial cells cannot translate mRNAs transcribed from human genes. d)Bacteri

Question

c)Bacterial cells cannot translate mRNAs transcribed from human genes. d)Bacterial ribosomes make errors when transcribing human mRNAs. 3)The human gene for a protein hormone has been isolated. Scientists want to produce this hormone. After successfully introducing the gene on a plasmid, the introduce this gene into a bacterial host, modifying the bacteria so that it can be used to bacteria produced the protein hormone, but the protein was defective, longer than normal, with intervening stretches of amino acids not found in the native protein. Which solution would be most likely to resolve this problem? A)Treat the defective protein with enzymes. B)Introduce human ribosomes into the bacteria. C) Start over, introducing a complementary DNA (cDNA) copy of the gene into the bacterial host. D)Introduce human mRNAs into the bacterial host 4)Which of the following would be of LEAST value in DNA profiling? Ajmitotyping B)STR (short tandem repeat) analysis C)sequencing blood group alleles Djanalysis of SNPs (single nucleotide polymorphisms)

Explanation / Answer

3. Option D is correct.
Most human genes contain introns. Bacteria do not contain splicing machinery So, we have to introduce human mRNA/cDNA into bacteria in order to express a functional protein.

4. Option C is correct.
DNA profiling is performed to study genetic variation. Sequencing blood group alleles would reveal the blood group only.

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