Format Tools Add-ons Help All changes saved in Drive ormal textArial 1) Short in

Question

Format Tools Add-ons Help All changes saved in Drive ormal textArial 1) Short interfering RNAs affect gene expression in eukaryotes by: a) blocking replication of certain genes b) blocking transcription of certain genes. c) binding to mRNAS and preventing translation. d) binding to ribosomes, preventing the two subunits from joining. 2) The human gene for a protein hormone has been isolated. Scientists want to introduce this gene into a bacterial host, modifying the bacteria so that it can be used to produce this hormone. After successfully introducing the gene on a plasmid, the bacteria produced the protein hormone, but the protein was defective, longer than normal, with intervening stretches of amino acids not found in the native protein. What is the most likely explanation? a)The bacteria can't transcribe the human gene. b)The gene that was introduced contained introns. c)Bacterial cells cannot translate mRNAs transcribed from human genes. d)Bacterial ribosomes make errors when transcribing human mRNAs.

Explanation / Answer

Ans 1...C...binding to mRNA and preventing translation

The interving sequence in ques are called introns that are found in eukaryotic mRNA and these have to removed by splicing because they are non coding sequence and does not code for anything

Ans 2...B...the gene that was introduced contained introns

As already explained introns are non coding sequence which has to be removed.in bacterial cell there is no mechanism to remove these introns to there are intervening stretches of amino acids found here which were not present in native protein because there in humans have the Mechanism to remove these introns and produce an effective protein

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