1. What would be the net ATP yield from complete aerobic oxidation of a triacylg
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Question
1. What would be the net ATP yield from complete aerobic oxidation of a triacylglycerol of 18:0 at sn1, 14:0 at sn2 and 16:0 at sn3.Show all of your steps, reactions and math to come up with these numbers. Do not forget mobilization and activation. Extra consideration (credit) if you include the fate of glycerol in your answer. (you will need to look for and back for this) 2. What would be the difference in net ATP output from the catabolism of 18:2912 fatty acid in comparison to an 18:0 fatty acid? Explain the difference and explain why there is a difference in ATP What did you find out? Show all steps in answer to each question, in detail.Explanation / Answer
For 14:0 fatty acid
Step 1.- Number of Carbons/2 = Number of Acetyl CoA formed.
=14/2=7 Acetyl CoA formed
Step 2.- Number of rounds in the Beta-oxidation necessary for converting the whole fatty acid to Acetyl Co A units: Number of Acetyl CoA minus 1 [(n/2)-1]
=(14/2)-1=6
Step 3 . If we consider that each NADH yields 2.5 ATP and each FADH2 yields 1.5 ATP then multiply the number of rounds times 4 and multiply the number of Acetyl CoA times 10
(6 x 4) + (7 x10)=94
Step 4.- Take two ATP that were used for the activation of the Fatty Acid
Total: 92 ATP
For 16:0 fatty acid
Step 1.- Number of Carbons/2 = Number of Acetyl CoA formed.
=16/2=8 Acetyl CoA formed
Step 2.- Number of rounds in the Beta-oxidation necessary for converting the whole fatty acid to Acetyl Co A units: Number of Acetyl CoA minus 1 [(n/2)-1]
=(16/2)-1=7
Step 3 . If we consider that each NADH yields 2.5 ATP and each FADH2 yields 1.5 ATP then multiply the number of rounds times 4 and multiply the number of Acetyl CoA times 10
(7 x 4) + (8 x10)=108
Step 4.- Take two ATP that were used for the activation of the Fatty Acid
Total: 106 ATP
For 18:0 fatty acid
Step 1.- Number of Carbons/2 = Number of Acetyl CoA formed.
=18/2=9 Acetyl CoA formed
Step 2.- Number of rounds in the Beta-oxidation necessary for converting the whole fatty acid to Acetyl Co A units: Number of Acetyl CoA minus 1 [(n/2)-1]
=(18/2)-1=8
Step 3 . If we consider that each NADH yields 2.5 ATP and each FADH2 yields 1.5 ATP then multiply the number of rounds times 4 and multiply the number of Acetyl CoA times 10
(8 x4) + (9 x10)=122
Step 4.- Take two ATP that were used for the activation of the Fatty Acid
Total: 120 ATP
The net ATP yield of the triacylglycerol is simply the sum of the ATP yield of the three fatty acids:
= 92+106+120= 318 ATP
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