at 12 krpm tiot Post lab questions an b 1. Which plates should be compared to de
ID: 133981 • Letter: A
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at 12 krpm tiot Post lab questions an b 1. Which plates should be compared to determine if any genetic transformation has occurred? Why? 2. What is meant by a control plate? What purpose does a control serve? 3. What's Glowing in E. coli cell when exposed to UV light? 4. What is the function of Arabinose? 5. What is the transformation efficiency of your competent E. coli cell? 6. Scientists prepare competent E.coli cells having a transformation efficiency of about 10 cells'ug plasmid DNA. Compare your competent E. coli cells with those prepared by sci 7, Calculate % score of your E. coli transformation efficiency (TE). Your T.E. 2s ?.?. of professionals (108) % score- X 100 53Explanation / Answer
1.genetic transformation means acquiring a new gene in the cells of bacteria.In order to determine the cell is transformed we need to keep the cell in a particular enviroment.For example if we want to check transformation of antibiotic resistant gene then we need to place the cells in the medium containing the antibiotic.If the cells had transformed then they will grow on the medium containing antibiotic, if not then the cells will not survive.
So to compare, the cells grown on normal agar should be compared with the cells grown on antibiotic medium.
2.control plates are used for comparison of experimental results.A positive control will be showing intact plasmid carrying the desired selectable marker (e.g., antibiotic resistance) and negative control will be showing the cells only no DNA.Negative and positive controls should be included in the transformation step to evaluate the success of the experimental procedure.
3.radiolabelled plasmid will be glowing under UV light.
4.it acts as sole carbon source inducing arabinose operon.
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