You have prepared lipid vesicles (spherical lipid bilayers) that contain Na-K pu

Question

You have prepared lipid vesicles (spherical lipid bilayers) that contain Na-K pumps as the sole membrane protein. All of the Nar-K pumps are oriented in sucha way that the portion of the molecule that normally faces the cytosol is on the inside of the vesicle and the portion of the molecule that normally faces the extracellular space is on the outside of the vesicle. Assume that each pump transports one Na ion in one direction and one K ion in the other direction during each pumping cycle (see Figure below for how the Na*-K pump normally functions in the plasma membrane) (20 points). 3) reieaing Naf ATP SPACE Na" CYTOSOL phosphate in high-energ linkage 3 naling Predict what would happen in each of the following conditions: A. The solutions inside and outside the vesicles contain both Na and K-ions B. The solution outside the vesicles contains both Na and K ions; the solution C. but no ATP. inside contains both Na and K ions and ATP. C. The solution outside contains Na;the solution inside contains Na and ATP

Explanation / Answer

The Na+ bind to the pump and cause the ATPase activity to hydrolyze ATP. The hydrolyzed ATP give free phosphate that transfer onto the pump. After attaching of phosphate group a conformational change in channel will occur, and lead to release of Na+ from the vesicle. However, outside the vesicle there is no K+ found, so that the pump will get stuck at that step and next steps of the cycle will not occur.

OBSERVE STEP 2 AND 3

GIVE THUMB'S UP

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