E.Coli Results SIM (sulfur, indole, motility) Online Resuts- E Coli does not hav
ID: 133450 • Letter: E
Question
E.Coli Results SIM (sulfur, indole, motility) Online Resuts- E Coli does not have the ability to produce hydrogen sulfide, negative result E Coli has the ability to produce indole, positive result E Coli is motile. Rationale- E Coli cannot reduce sulfur E Coli contains the enzyme tryptophanase which splits up the amino acid, tryptophan The result of this is indole E Coli has flagella allowing them to move around 2 MRVP (Methyl Red and Voges-Prosakuer) Online Results E. Coli is positive for MR (red) E Coli is ne gative for VP (yellow) Rationale- E Coli has the ability of performing a mixed acid fermentation of glucose. E Coli cannot further metabolize pyruvic acid to form acetyl-methyl carbinol (acetoin). 3 Nutrient Gel Online Results E Coli has positive growth Nutrient gel supports the growth of non-fastidious cr complex or special diet for growing and was therefore able to grow Rationale- ganisms.E.Coli does not require aExplanation / Answer
The sulfur- indole-motility tests are very correct. E.coli is a motile, flagellated organism and has the tryptophanase enzyme which breaks down tryptophan to produce indole. Also, E.coli sulfur negative and hence it cannot reduce hydrogen sulfide. Yes, correct. E.coli bacteria is MR(methyl red test) positive and VP (Vogues Proskauer test) negative. MR test checks the ability of bacteria to produce glucose from mixed acid fermentation while VP test determines the ability of an organism to produce acetyl methyl carbinol from glucose fermentation. E.coli bacteria has the ability to produce both the products of the tests. Nutrient agar or gel is a simple medium mainly containing yeast/beef extract and some salts. This medium is used for growing non-fastidious organisms which do not require any special supplementation for their growth.
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