You are trying to characterize isolates that you suspect have the ability to deg

Question

You are trying to characterize isolates that you suspect have the ability to degrade 2,4-D. you isolate two strains and begin to characterize them using molecular techniques. You screen for the tfd-b gene (one of the genes that encodes for an enzyme responsible for 2,4-D degradation) using seminested PCR, generate ERIC PCR fingerprints and do a plasmid profile to look for the large 80 kb plasmid which carries the 2,4-D genes. Using the well-studied 2,4-D degrader Ralstonia eutropha as a control, what can you say about each isolate based on the results shown?

Environmental Microbiology Chapter 13 question #8

281 Seminested PCR Eric PCR 2 1 2+ Plasmid Profile L = 123 bp ladder 1 = strain 1 2 = strain 2 = negative control + = positive control (Alcaligenes eutrophus)

Explanation / Answer

According with the seminested PCR both strains have the tfd-g gene. Also according with the plasmid profile both strains have the large 80 kb plasmid which carries the 2,4-D genes in the positive control (Ralstonia eutropha). And according with the ERIC-PCR strain two has the enterobacterial repetitive intergenic consensus sequences similar to Ralstonia eutropha which means they’re probably phylogenetically related. On the contrary according with the ERIC profiles strain one isn’t related to Ralstonia eutropha.

Related Questions

Navigate

• Browse (All)
• Browse Y
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.