How can both Southern and Western blotting assist with genetic recombination exp
ID: 133015 • Letter: H
Question
How can both Southern and Western blotting assist with genetic recombination experiments?
Both methods will confirm that the gene is in the plasmid before it is transfected into cells.
One will identify the specific DNA in the plasmid, and the other will identify the specific protein part of the plasmid.
One is used to recombine the plasmid and the other is used to identify the gene sequences in the plasmid.
One will identify a specific DNA sequence in transformed cells and one will identify expression of the protein from that sequence.
Explanation / Answer
Southern blot helps to check the presence of a specific DNA sequence in the given DNA and western blot helps to check the expression of the desired protein in the pool of protein.
So the correct answer is option D- One will identify a specific DNA sequence in transformed cells and one will identify expression of the protein from that sequence.
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