1. A primer is A short fragment of double stranded DNA that binds to specific DN
ID: 132679 • Letter: 1
Question
1. A primer is A short fragment of double stranded DNA that binds to specific DNA regions and initiates replication A short fragment of single stranded DNA that binds to specific DNA regions and initiates replication A short polypeptide that binds to specific DNA regions and initiates replication A short fragment of single stranded DNA that binds to specific DNA regions and serves as a starting point in DNA synthesis a. b. c. d. 2. What type of PCR did we use in the APOE haplotyping project? a. Digital PCR b. Quantitative PCR c. End-point PCR d. RT-qPCR In PCR, the number of the target DNA fragment grow exponentially. The first two intact target DNA fragments are produced at the end of the 3. a. 1tcycle b. 2nd cycle C. 3"d cycleExplanation / Answer
1. In this question the option d is absolutly correct because the primer is single strand DNA fragment which is used to synthsize DNA in Vitro due to the activity of DNA polymerase only on exsisting DNA fragements. However here the option a and b is practicaly right because the in case PCR (polymerase chain reaction) the amplification or replication of initial DNA fragement requires primer and since in each strand there will be replication hence sigle strand primer will become double strand primer during the process of replication.
2. APOE haplotype also known as apolipoprotein haplotyping which is known to get the information on human longivety gene. This is done mostly by using RT qPCR (Quantitative reverse transcription PCR?). The RTqPCR is known to use for RNA to DNA aplification, in order to know the unknown proterin produced by unknown gene.
3. The target DNA will be produced in next cycle of PCR which means the 1st cycle DNA oligonuclotide will be the final DNA target molecule and this target molecule will serve as primer for next cycle and so no. This is happining in PCR because in each cycle of PCR template denaturation, primer annealing and primer extension repeates.
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