Brian becomes interested in identifying some of his skin normal flora. He streak

Question

Brian becomes interested in identifying some of his skin normal flora. He streaks a plate for isolation and subcultures a tiny yellow colony. In order to learn more about the bacteria that form this colony, he places it into a drop of hydrogen peroxide The bacterium bubbles profusely. To which conclusion/s does Brian come? This normal flora bacterium is equipped with the enzyme catalase and can thus detoxify hydrogen peroxide as shown in the following reaction: 2 H2O2 2H2O + O2 This normal flora bacterium is killed by small amounts of hydrogen peroxide. This normal flora bacterium is likely tailed by small amounts of superoxide in its environment. This normal flora bacterium is a strict anaerobe Following is a picture of the enzyme that we spoke about in class, superoxide dismutase: This enzyme stabilizes O2 and H* reactants such that the reaction is more likely to proceed recognizes thousands of diverse substrates in addition to superoxide. undergoes a permanent change in conformation after catalyzing the formation of H2O2 and O2. increases the energy needed to form the transition state complex.

Explanation / Answer

(1) A As shown when hydrogen peroxide is decomposed it yields water and oxygen gas, which is the bacterium that bubble profusely. (2) D (A) Wrong: hydrogen peroxide or other superoxides are catalyzed, not the ions. (B) Wrong: biological enzymes have a high specificity. Superoxide dismutase only binds with itself and a few superoxides or radicals (C) Wrong: Enzymes never undergo permanent changes in conformation

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