You have lysed several different cell lines in order to obtain the proteins from

Question

You have lysed several different cell lines in order to obtain the proteins from these cells. You have performed a BCA assay to quantitate the amount of protein in your cell lysates. You now wish to load 20,00 ug (micrograms) of each cell lysate into the wells of a polyacrylamide gel for electrophoresis. Calculate the volume of each lysale you should load into the wells if each lysate has the following concentration (as determined from the BCA Assay). Show your work for at least one of the calculations in the space below the table.

Explanation / Answer

1.0525mg/mL = 1.0525 ug/uL
(20ug)(uL/1.0525ug)=19.00uL

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