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I was absent for the lab about an experiment of Chromatography. I just collected

ID: 12245 • Letter: I

Question

I was absent for the lab about an experiment of Chromatography. I just collected data of the experiment which my friend got. Could you help me answer the 2 following questions by looking at my data, please? I will be very appreciate if you detailed your answers so that I can understand.

2. How polar are each of the pigments? Be specific.

3. Why did the manufacturers choose the pigments for their pens? Hint: Think of practical applications.

 SEPARATION OF MIXTURES: CHOMATOGRAPHY

Paper chromatography is a technique used to separate the components present in a mixture. Two basic concepts underlie this process; capillary action and solubility differences of the components.

You will use paper chromatography to separate the pigments that make up black or blue or red ink in two different pens: Paper mate® (ball point pen) and Sharpie® (permanent maker).

Set up: Obtain 6 pieces of chromatography paper. Using a pencil, mark a line 3 cm from one edge. On the line, draw a dot using each pen in the order listed above. See Figure 7 below. Place paper in the first solvent, water.

Make sure the solvent volume is below the pencil line. Incubate for 10 minutes and record your observations in the data table. Repeat for the rest of the solvents.

I attach the table of data and result

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Explanation / Answer

Your picture didnt load but.... The more soluble a molecule is, the higher it will migrate up the paper If the sample contains more than one color, that means it must have more than one kind of molecule.The stationary phase is usually a piece of high quality filter paper. The mobile phase is a developing solution that travels up the stationary phase, carrying the samples with it. Components of the sample will separate readily according to how strongly they adsorb on the stationary phase versus how readily they dissolve in the mobile phase.

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