If a primary antibody against human tubulin was made in a rabbit, will a goat an
ID: 11757 • Letter: I
Question
If a primary antibody against human tubulin was made in a rabbit, will a goat anti-rabbit secondary antibody work?1. No – a goat anti-rabbit antibody will not target human proteins such as human tubulin.
2. Yes – The primary and secondary were both made in the same organism so they are compatible.
3. Yes – The secondary antibody will correctly target the primary anti-tubulin.
4. No – the primary antibody targets tubulin but the secondary antibody does not.
5. No – The secondary antibody must target tubulin to work.
Explanation / Answer
If a primary antibody against human tubulin was made in a rabbit, will a goat anti-rabbit secondary antibody work?
1. No – a goat anti-rabbit antibody will not target human proteins such as human tubulin.
2. Yes – The primary and secondary were both made in the same organism so they are compatible.
3. Yes – The secondary antibody will correctly target the primary anti-tubulin.
4. No – the primary antibody targets tubulin but the secondary antibody does not.
5. No – The secondary antibody must target tubulin to work.
Note: both #2 and #3 are technically accurate, though #2 is the "best answer"
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