Which, if any, of the following would not be a step in cloning DNA using a plasm
ID: 11519 • Letter: W
Question
Which, if any, of the following would not be a step in cloning DNA using a plasmid vector like pUC19?A. Cut DNA and plasmid with the same restriction endonuclease
B. Join DNA and plasmid DNA with ligase
C. Insert recombinant plasmid into receptive bacterial cells
D. Package the ligated DNA into phage bodies and infect bacteria
E. All are steps used in pUC19 plasmid cloning
Which, if any, of these statements is not true regarding a cloning vector?
A. it contains an origin of replication
B. it could be derived from a phage or plasmis
C. allows for the selection of recombinant vectors that contain inserts
D. can enter and multiply within a living cell
E. All of the above are true
Explanation / Answer
The first answer is D as phage vectors are not needed to transfer DNA to cells, when plasmid vectors are already involved. As all of the statements for the second question are true, the answer is E
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