AaBbCcDdE AaBbCcDdE Normal List Paragraph Gas Chromatography 1. What is retentio

Question

AaBbCcDdE AaBbCcDdE Normal List Paragraph Gas Chromatography 1. What is retention time in gas chromatography? 2. Define 'Number of theoretical plates 3. Name three variables that affect retention times in gas chromatography 4. What are the commonly used carrier gases in gas chromatography analysis when using Flame ionization detector? What is the effect on resolution due to slow injection of a large sample volume? Fill in the following blanks using the words included in the word bank: injector, detector, mobile phase, stationary phase. a. In gas chromatography an inert gas is used to carry the sample through the column 5. 6. to the detector; this gas is known as the b. The will sense the compounds as they exit the GC, this will pick up the color change in the flame due to the Beilstein reaction. The packing used in a GC, known as the aids in the separation of compounds due to the varying adsorption of molecules on the packing. Compounds are introduced to the column by way of the c. d. which is sometimes heated to aid in the vaporization of the sample before it enters the column. 7. Use the following gas chromatogram of a mixture of dioxane and cyclohexane eluted on a non-polar column to answer the following question. 1-t-rio sane time a. What is the identity of the substance labeled peak A? Explain how you deduced this.

Explanation / Answer

A1. Retention time is the quantity used to measure the time taken by a species to pass through the gas chromatography column. That is basically a peak which appears once the solute is eluted out. This time is started when injection happens and till the solute passes the column.

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