The proteins are incubated with the primary enzyme. The proteins are transferred

Question

The proteins are incubated with the primary enzyme. The proteins are transferred from the gel onto a thirn membrane of nitrocellulose or other absorbing compound. rst The proteins are sprayed from a capillary under a strong electric field. Second The proteins are incubated with the secondary antibody Third Proteins reacted with substrate for secondary antibody enzyme tag. Fourth The proteins are digested using enzymes. Fifth The proteins are incubated with the primary antibody The separation of proteins via electrophoresis. ldentify the main procedures involved in a western blot and the correct order in which they are performed. (Not all the procedures in the eft hand column are necessary for your answer.) how transcribed image text

Explanation / Answer

1. The proteins are digested using enzymes

2. The separation of proteins via electrophoresis

3. The proteins are transferred from the gel onto a thin membrane of nitrocellulose or other absorbing compound

4. The proteins are incubated with primary antibody

5. The proteins are incubated with the secondary antibody

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