3. Transfer the contents of the beaker into a 15 mL plastic centrifuge tube The

Question

3. Transfer the contents of the beaker into a 15 mL plastic centrifuge tube The mixture will separate into two different layers. The glycerol will sink to the bottom, and the methyl ester (biodiesel) will float to the top. Allow the mixture to cool and then place it in a centrifuge and spin for another 3 minutes (don't forget to counterbalance the centrifuge). If the layers have not separated continue to centrifuge for another 3 minutes What properties of two layers are different that they separate? what properties of two layers are different that one layer sinks to the bottom? notriotod to nrenare GCMS vial Mix 4mL of CH2C12 with one drop of biodiesel (Make sure n

Explanation / Answer

1) It is due to Interfacial Tension and density

2) The one which is on the bottom have higher density than the other. The more denser one always liew on the bottom. So here glyserol is denser than the methyl ester, and so sinks to bottom

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