11. The sequence of a 29 aa long peptide can be determined from the following da

Question

11. The sequence of a 29 aa long peptide can be determined from the following data: a) Treatment of the peptide with Sanger's reagent (FDNB) reveal Val. Which terminus is thist N-trminus or C-terminus)2 that a terminal amino acid is 1. n s (1 pt) n with an enzyme, separation of peptides, and Edman degradation give the sequences of peptide fragments shown below. Which enzyme was used?l pt) VGAHAGEYGAEATE AAWGK VLSPAK TNVK c) Digestion with an enzyme, separation of peptides, and Edman degradation give the sequences of peptide fragments shown below. Which enzyme was used? GAEATE GKVGAHAGEY VLSPAKTNVKAAW (1 pt) d) Write the sequence of the 29 aa peptide. (5 pts)

Explanation / Answer

a) N-terminus

b)Trypsin, as the cleavage has occurred from lysine

c) Chymotrypsin, as the cleavage has occurred from Tyrosine and tryptophan

d) amino acid =

VLSPAKTNVKAAWGKVGAHAGEYGAEATE

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