You wish to prepare 250. mL of a 0.300 M Tris buffer at room temperature (25 C)

Question

You wish to prepare 250. mL of a 0.300 M Tris buffer at room temperature (25 C) such that it has a pH of 7.80 at 10.0 C.
Starting with Tris base (F.W. 121.2, 99.9 % purity), answer the following questions regarding the preparation of this buffer. Refer to Table 2-1 in your coursepack for information regarding the temperature coefficient of Tris (pH/T).

-What mass of Tris is needed?

-To what pH (at room temperature, 25 C) would you adjust the buffer so that it had a pH of 7.80 at 10.0 C?

-Which would be the correct solution to use to adjust the pH?

-What volume of the 2 M solution identified above will be needed to reach the correct pH?

Explanation / Answer

You wish to prepare 250. mL of a 0.300 M Tris buffer at room temperature (25 C)

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