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ID: 1077523 • Letter: I
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For the following 3 buffer procedures, calculate how much of each ingredient is needed. For each buffer procedure you will have a solid (g) ingredient, a liquid (mL) ingredient, and water Show all work Procedure 2 Prepare 50 mL of acetic acid buffer at strength of 0.1 M and at a pH of 5.0 using 1 M acetic acid, sodium acetate, and water Procedure 3 Prepare 50 mL of TRIS buffer at strength of 0.1 M and at a pH of 7.5 using Tris, 1 MHCI and water Formula weight (g/mol) 121.1 Buffer pKa TRIS 8.3 Calculate the weight of the solid buffer you would need to make 50 mL of 0.1 M solution. Procedure 3: 0.602g Tris In 20ml Record pH Procedure 2: 0.262g Sodium Acetate Add HCI dropwise until a pH of7 1.8ml Acetic Acid Total volume 50m Bring up to 50ml Record pH Compare to 4.31ml HCl calculatedExplanation / Answer
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