cThe fluorescence of each of a series of acidic solutions of quinine was determi
ID: 1074134 • Letter: C
Question
cThe fluorescence of each of a series of acidic solutions of quinine was determined five times. The results are given below.
Concentration, ng ml-1
0
10
20
30
40
50
Fluorescence intensity(arbitrary units)
4
22
44
60
75
104
3
20
46
63
81
109
4
21
45
60
79
107
5
22
44
63
78
101
4
21
44
63
77
105
Determine the slopes and intercepts of the unweighted and weighted regression lines. Calculate, using both regression lines, the confidence limits for the concentrations of solutions with fluorescence intensities of 15 and 90 units. Compare the results of the unweighted regression to those of weighted regression.
Concentration, ng ml-1
0
10
20
30
40
50
Fluorescence intensity(arbitrary units)
4
22
44
60
75
104
3
20
46
63
81
109
4
21
45
60
79
107
5
22
44
63
78
101
4
21
44
63
77
105
Determine the slopes and intercepts of the unweighted and weighted regression lines. Calculate, using both regression lines, the confidence limits for the concentrations of solutions with fluorescence intensities of 15 and 90 units. Compare the results of the unweighted regression to those of weighted regression.
Explanation / Answer
Slope = (Y2 -Y1)/ (X2-X1)YY
Y axis is fluorescence intensity and X axis is concentration in ng per mL
Slope = (44-22)/(20-10) from 1st experimental data
Slope = 2.2
Intercept = value of intensity which touch y axis, at concentration,the value of intensity is 4
Hence, Intercept = 4
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