Reversed-phase chromatography uses a A. Polar stationary phase and less polar mo
ID: 1067796 • Letter: R
Question
Reversed-phase chromatography uses a A. Polar stationary phase and less polar mobile phase. B. Less polar stationary phase and more polar mobile phase. C. Constant mobile phase composition. D. Continuous change of solvent polarity during elution. E. Continuous temperature change during elution. Reversed-phase chromatography uses a A. Polar stationary phase and less polar mobile phase. B. Less polar stationary phase and more polar mobile phase. C. Constant mobile phase composition. D. Continuous change of solvent polarity during elution. E. Continuous temperature change during elution. A. Polar stationary phase and less polar mobile phase. B. Less polar stationary phase and more polar mobile phase. C. Constant mobile phase composition. D. Continuous change of solvent polarity during elution. E. Continuous temperature change during elution.Explanation / Answer
Answer is B.
Less polar stationary phase and more polar mobile phase.
That's why it is called reverse, as in normal chromatography we use highly polar silica or alumina as stationary phase
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