22-28 Chromatograms of compounds A and B were obtained at the same flow rate wit

Question

22-28

Chromatograms of compounds A and B were obtained at the same flow rate with two columns of equal length. Which column has more theoretical plates? Which column has a larger plate height? Which column gives higher resolution? Which column gives a greater relative retention? Which compound has a higher retention factor? Which compound has a greater partition coefficient? What is the numerical value of the unadjusted relative retention? Why does plate height depend on linear flow rate, not volume flow rate?

Explanation / Answer

From the chromatographic plot

(a) Column A has more theoretical plates which gives better resolution.

(b) Column B has larger plate height.

(c) Higher resolution with column A

(d) column B gives greater relative retention.

(e) compound B has greater retention factor

(f) compound A has greater partition coefficient

(g) Retention times for compound A = 8

compound B = 10

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