The protein apotransferrin binds two Fe(lll) ions for cell transport. Consider w
ID: 1062848 • Letter: T
Question
The protein apotransferrin binds two Fe(lll) ions for cell transport. Consider when a 2.00 mL solution of apotransferrin is titrated with 102 mu L of a 1.38 M Fe(lll) solution. The initially colorless solution turns red, and the color change can be measured with a spectrophotometer. First, explain why there is a difference in spectrophotometric response during titration before and after the equivalence point by placing the statements below into the appropriate categories. Now, calculate the concentration of apotransferrin present in the original 2.00 mL solution.Explanation / Answer
Solution.
given that
102 micro ltrs of 1.38 M Fe (111) solution is utilized
1 micro liter = 10^-6 L
Moles of Fe (111) utilized =Molarity*valume
=1.38*102*10^-6
= 1.4076*10^-4 mol
we know that 2 Fe(111) ion bond to 1 apotransferrin
So, 1.38*10^4 mole of Fe(111) ion bind to 8.772*10^-5 mol
Thus, mole of apotransferrin = 8.772*10^-5 mol
valume of apotransferrin =2ml =2*10^-3 L
Molarity = MOLes/ Volume = 8.772*10^-5/2*10^-3
= 0.04386 M
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