L Experiment #10 Pre-Lab Exercise What is an enzyme? Give a description of what
ID: 1061343 • Letter: L
Question
L Experiment #10 Pre-Lab Exercise What is an enzyme? Give a description of what an enzyme is terms of its chemical in composition (what kind of biomolecule) and in terms of what it does (ts function). En mes ane Cata acce A chemical reaction catalyzed by the enzyme acid phosphatase is shown in the How would you classify this enzyme in terms of the enzyme classification scheme described in the text book, i.e.. oxidoreductase, transferase, hydrolase, lyase, isomerase, or ligase? List at least four factors that can influence enzyme activity and indicate what is meant by the term "enzyme activity" in this context (see the text book). Considering the biochemical (protein) nature of enzymes, what are some factors or chemical and physical agents that can destroy or denature enzymes? Consider the earlier experiment regarding properties of proteins. You should list at least three.Explanation / Answer
Q1
Enzymes: they are proteins that go about as impetuses inside living cells. Impetuses increment the rate at which synthetic responses happen without being expended or for all time changed themselves. A synthetic response is a procedure that proselytes at least one substances (known as reagents, reactants, or substrates) to another kind of substance (the item). As an impetus, a catalyst can encourage a similar compound response again and again.
Like all proteins, compounds are made out of at least one long chains of interconnected amino acids. Every chemical has a novel grouping of amino acids that causes it to overlap into a trademark shape.
Normal proteins delivered in minor amounts by every living creature (microscopic organisms, plants, and creatures) and working as very particular biochemical impetuses in changing over one particle into another. Catalysts are basic to life since they accelerate metabolic responses to an exceptionally incredible degree, however don't experience any adjustment in themselves. In the business they are utilized for debasing oil slicks and squanders into safe mixes, in cleaning fat (sustenance) stains, and in aging procedures to make mixed refreshments. Proteins are exceptionally delicate to natural conditions and capacity best just inside a restricted scope of temperature and corrosiveness (pH) levels.
On the surface of every protein is an exceptional split called the dynamic site, which gives a place where reagents can "meet" and collaborate. Much like a bolt and its key, a protein's dynamic site will just oblige certain reagents, and just a single sort of substance response can be catalyzed by a given compound.
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Q2
phosphatase -----hydrolase
It is a protein that expels a phosphate aggregate from its substrate by hydrolysing phosphoric corrosive monoesters into a phosphate particle and an atom with a free hydroxyl assemble.
This activity is straightforwardly inverse to that of phosphorylases and kinases, which append phosphate gatherings to their substrates by utilizing fiery particles like ATP. A typical phosphatase in numerous living beings is basic phosphatase. Another extensive gathering of proteins present in archaea, microbes, and eukaryote shows deoxyribonucleotide and ribonucleotide phosphatase or pyrophosphatase exercises that catalyze the disintegration of dNTP/NTP into dNDP/NDP and a free phosphate particle or dNMP/NMP and a free pyrophosphate particle. The other gathering of phosphatase is on the whole called as protein phosphatase, which expels a phosphate aggregate from the phosphorylated amino corrosive buildup of the substrate protein. Protein phosphorylation is a typical posttranslational change of protein catalyzed by protein kinases, and protein phosphatases invert the impact.
lkaline phosphatase is a hydrolase catalyst in charge of expelling phosphate bunches from many sorts of atoms, including nucleotides, proteins, and alkaloids. The way toward evacuating the phosphate gathering is called dephosphorylation.
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