The effect of transition metals on the binding of HPRG was investigated next. Th

Question

The effect of transition metals on the binding of HPRG was investigated next. The ability of increasing concentrations of Cu and Zn to promote HPRG binding to heparin at pH = 7.3 was 2+ 2+ measured. The results are shown in Figure 2.4. In addition, the binding of HPRG to heparin in the presence of these ions was compared at various pH’s. Figure 2.5 shows the comparison of binding at pH = 6.0 and at pH = 7.4 in the presence of 5.2 nM zinc. What is your interpretation of these results?

400 300 Binding 200 (units) 100 0.1 1.0 Cation concentration M) Cu Zn Figure 2.4: Binding of HPRG to heparin in the presence and of copper and zinc ions (based on Borza and Morgan, 1998) 700 600 500 Binding 400 (units) 300 200 100 1.0 2.0 No Zn OWith Zn Figure 2.5: Binding of HPRG to heparin in the presence and absence of 2.5 nM zinc ions at two different pH values (based on Borza and Morgan, 1998).

Explanation / Answer

1. According to Figure 2.4, In intial stages, at lower concentration of two transition metals have no effect on binding of HPRG to haparin but increase concentration of Cu and Zn increases in HPRG binding towards haparin. Zinc is more effective in compare with Cu

2. According to Figure 2.5, At pH= 6 the binding of HPRG to haparin in presence as well as in absence of Zn ions increases with increase in concentration ,where as at pH =7.4. the binding ofHPRG to haparin, in absence of Zn ions has no effect it remains unchanged but in presence of Zn ions there is good increase in binding but it is less compared to pH= 6

Related Questions

Navigate

• Browse (All)
• Browse T
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.