Suppose you are trying to separate a mixture of compounds using reversed-phase l

Question

Suppose you are trying to separate a mixture of compounds using reversed-phase liquid chromatography (RPLC). On the first run, using a mobile phase containing 40% tetrahydrofuran and 60% water, the peaks all elute between 0 and 3 min and are too close together to be quantitatively resolved. To improve the resolution of the peaks, should you increase or decrease the amount of tetrahydrofuran in the mobile phase for the next run? not enough information increase decrease In a separate experiment, you are trying to separate a mixture of compounds using normal-phase liquid chromatography (NPLC). On the first run, with a mobile phase containing 35% toluene and 65% acetonitrile, the peaks again eluted quickly and were poorly resolved. To improve the resolution of the peaks, should you increase or decrease the amount of acetonitrile in the mobile phase for the next run? increase decrease not enough information

Explanation / Answer

Answer:-

1. THe amount of tetrahydrofuran should increase in the mobile phase for the next run.

2 the amount of acetonitrile in the mobile phase should increase in the next run to improve the resolution of the peaks.

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