H_3 C - O - C(CH_3)_3 Methyl t-butyl ether (MTBE), shown above, is a controversi
ID: 1055112 • Letter: H
Question
H_3 C - O - C(CH_3)_3 Methyl t-butyl ether (MTBE), shown above, is a controversial gasoline additive. Of the following analytical techniques, which would be the best method to measure quantitatively trace amounts of MTBE in contaminated groundwater? Capillary electrophoresis Gas chromatography Atomic absorption spectroscopy Fluorescence spectroscopy The key components common to both a high performance liquid and a gas chromatographic system include all of the following EXCEPT a detector stationary phase device for temperature programming sample injectorExplanation / Answer
Methyl t-butyl ether (MTBE), shown above, is a controversial gasoline additive. Of the following analytical techniques, which would be the best method to measure quantitatively trace amounts of MTBE in contaminated groundwater? (
A) Capillary electrophoresis
(B) Gas chromatography
(C) Atomic absorption spectroscopy
D) Fluorescence spectroscopy -ANSWER
Fluorescence spectroscopy (otherwise called fluorometry or spectrofluorometry) is a sort of electromagnetic spectroscopy that dissects fluorescence from a specimen. It includes utilizing a light emission, normally bright light, that energizes the electrons in particles of specific mixes and makes them transmit light; regularly, however not really, unmistakable light. A reciprocal procedure is assimilation spectroscopy. In the unique instance of single atom fluorescence spectroscopy, force vacillations from the radiated light are measured from either single fluorophores, or sets of fluorophores.Molecules have different states alluded to as vitality levels. Fluorescence spectroscopy is essentially worried with electronic and vibrational states. By and large, the species being analyzed has a ground electronic express (a low vitality state) of intrigue, and an energized electronic condition of higher vitality. Inside each of these electronic states are different vibrational states.
The particle then drops down to one of the different vibrational levels of the ground electronic state once more, emanating a photon in the process. As atoms may drop down into any of a few vibrational levels in the ground express, the radiated photons will have distinctive energies, and along these lines frequencies. Thusly, by dissecting the diverse frequencies of light radiated in fluorescent spectroscopy, alongside their relative forces, the structure of the distinctive vibrational levels can be resolved.
For nuclear species, the procedure is comparative; be that as it may, since nuclear species don't have vibrational vitality levels, the transmitted photons are frequently at an indistinguishable wavelength from the occurrence radiation. This procedure of re-discharging the ingested photon is "reverberation fluorescence" keeping in mind it is normal for nuclear fluorescence, is seen in sub-atomic fluorescence also.
In a common fluorescence (emanation) estimation, the excitation wavelength is settled and the recognition wavelength changes, while in a fluorescence excitation estimation the discovery wavelength is altered and the excitation wavelength is shifted over an area of intrigue. A discharge guide is measured by recording the outflow spectra coming about because of a scope of excitation wavelengths and joining them all together. This is a three dimensional surface information set: outflow force as a component of excitation and discharge wavelengths, and is regularly delineated as a form outline
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GAS chromatographic system include
High performance liquid and g GAS chromatographic system include EXCEPT
a)detector
b)stationary phase
c) device for temperature programming--------answer
d) sample injector
Gas chromatography (GC) is a typical sort of chromatography utilized as a part of scientific science for isolating and breaking down exacerbates that can be vaporized without disintegration. Normal employments of GC incorporate testing the immaculateness of a specific substance, or isolating the distinctive parts of a blend (the relative measures of such segments can likewise be resolved). In a few circumstances, GC may help in distinguishing a compound. In preparative chromatography, GC can be utilized to get ready immaculate mixes from a blend.
In gas chromatography, the portable stage (or "moving stage") is a transporter gas, typically an idle gas, for example, helium or a lifeless gas, for example, nitrogen. Helium remains the most normally utilized transporter gas as a part of around 90% of instruments in spite of the fact that hydrogen is favored for enhanced separations.[3] The stationary stage is a tiny layer of fluid or polymer on a latent strong support, inside a bit of glass or metal tubing called a section (a respect to the fractionating segment utilized as a part of refining). The instrument used to perform gas chromatography is known as a gas chromatograph (or "aerograph", "gas separator").
The vaporous mixes being broke down communicate with the dividers of the segment, which is covered with a stationary stage. This causes every compound to elute at an alternate time, known as the maintenance time of the compound. The correlation of maintenance times is the thing that gives GC its logical value.
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