In reverse phase liquid chromatography which mixture below represents the greate

Question

In reverse phase liquid chromatography which mixture below represents the greatest mobile phase strength? 20% methanol with 80% toluene 100% methanol (CH_3OH) 80% toluene with 20% methanol 100% toluene (C_7H_8) When of the following probably will NOT help improve the separation of two peaks m reverse phase liquid chromatography? Choosing a longer column with the same plate height. Changing to a column with more theoretical plates Choosing a column with smaller particles in the stationary phase. Lowering the flow rate

Explanation / Answer

Question 2

In liquid chromatography, the mobile phase strength refers to the ability of the solvent (eluent) to elute compounds more quickly from the column. In reverse phase liquid chromatography, the stationary phase is non-polar in nature and the non-polar solvents have higher stregnth to eulte the compounds. Therefore 100% toluene has higher mobile stregth.

Question 3

The answer is flow-rate. The columns with small stationary particle size, the flow-rate does not have significant effect over the resolution.

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