Suppose you want to separate two amino acids by the technique described in Part

Question

Suppose you want to separate two amino acids by the technique described in Part A, but you find that they have identical R_f values. Which of the following would probably help solve the problem? Change mobile phase. Use longer pieces of paper and increase the spot migration distance. Use larger samples of amino acids. Explain your answer: ______________ Suppose that while performing Part B, you weigh the casein and filter paper before they are completely dry. How will this error influence the reported percentage of casein? Increase it. Decrease it Have no effect on it. Explain your answer: _____________

Explanation / Answer

a)

change mobile phase... all other will not helpt, since you are not exploiting the basics of TLC... you must change the affinity of amino acids toward solvent/stationary phase

longer piece will simply have a larger mark of Rf but will not separate them

larger sample of amino acids will oonly make spots larger, but will not be separated

changing solvent(mobilhe phjase) will have different interactions, therefore interact differently and increase Rf distance (i..e separations)

Q4

You will have a wet mix, therefore, the mass is falsely larger than the actual value... so it will have a higher error

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