The following kinetic data were obtained for enzyme which catalyzes the reaction

Question

The following kinetic data were obtained for enzyme which catalyzes the reaction. S_1 + S_2 = P_1+P_2 where S_1=NAD; S_2=CH_1CH_2OH, P_1 =CH_3CHO; P_2=NADH All possible combinations for equilibrium isotope exchanges were performed and no exchanges were observed (all were inhibited). Based upon the analysis of the data, answer the questions below. The kinetic mechanism is ordered sequential random sequential nonsequential insufficient data What is the K_n for S_1? What is the K_n for S_2? What is the V_max? The effect exerted by S_3 on the reaction relative to S_1 is allosteric positive modulation uncompetitive inhibition noncompetitive (mixed) inhibition competitive inhibition None of the preceding Illustrate the kinetic mechanism (line diagram. Cleland notation) and indicate the positions of S_1, S_2, P_1, and P_2

Explanation / Answer

From the given data above,

1. The kinetic mecahanism is,

B. random sequential

2. Km for S1 = 160 mM

3. Km for S2 = 400 mM

4. Vmax = 807 umol/min

5. Type of effect S2 has compared to S1,

D. competitive inhibition

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