1. Compare Beer\'s law with the equation of a line. Identify x (the independent
ID: 1047561 • Letter: 1
Question
1. Compare Beer's law with the equation of a line. Identify x (the independent variable), y (the dependent variable), m, and b using the terms in Beer's law2. What is a calibration curve? And how is it used in an experiment? 1. Compare Beer's law with the equation of a line. Identify x (the independent variable), y (the dependent variable), m, and b using the terms in Beer's law
2. What is a calibration curve? And how is it used in an experiment?
2. What is a calibration curve? And how is it used in an experiment?
Explanation / Answer
1.
Beer's law equation : A = cl
straight line equation: y = mx + b
x = the independent variable = c (concentration)
y = the dependent variable = A (absorbance)
m = slope = x l = (for unit cell length, l = 1)
b = intercept = 0
2.
A calibration curve is a method for determining the unknown concentration of a substance in an sample by comparing standard samples of known concentration to the unknown
At first a set of experiments are performed to obtain the absorbance (A) value of set of known concentrations (c). Then A is plotted against c to get a standard calibration curve which is a straight line passing through origin for Beer's law experiment. Then when a unknown concentration is need to know of that particular sample, just measure the absorbance (A) value of the unknown sample and evaluate the concentration from the calibration curve.
Related Questions
Navigate
Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.