Umboratory Asšignment 1. Figure 3 shows a HPLC chromatograph of an analyzed samp
ID: 1046907 • Letter: U
Question
Umboratory Asšignment 1. Figure 3 shows a HPLC chromatograph of an analyzed sample that contained 3-nitrophenol, benzophenone butylparaben, ethylparaben, and ketoprofen. The HPLC utilized a Waters Acquity BEH C-18 column, with a length of 100 mm, and the mobile phase was 60% water and 40% acetonitrile. Determine the number of plates, the height of equivalent theoretical plates, and the resolution of the elution from the chromatograph shown. (For the resolution calculation, use the peaks corresponding to 3-nitrophenol and benzophenone.) Figure 3: Chromatograph of a sample that contains the compounds shown. The retention time and standard deviation of each peak is shown with the name and structure of the compound. ketoprofen 19446 min 0.01532 min ? ethylparaben 1.0757 min S-nitrophenol 30.9724 min butylparaben t 3.1645 min ? 0024S2 min ? 7820 x10 Jmin benzophenone t 46718 min E 0.03659 min Time (min.)Explanation / Answer
For finding the number of plates(N) ,formula is:
Where,
, is retention time
, is peak width
Where
= standard deviation
Average retention time :
Average standard deviation
On putting values in formulae
For finding the height of equivalent theoretical plates (H)
Where
L, is length of the column
N, number of plates
On putting the values in formula
for finding resolution(R):
On putting values
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