Jump to. iversity-CHEM 332-Spring18-GOOD > Activities and Due Dates Ch23 /21/201

Question

Jump to. iversity-CHEM 332-Spring18-GOOD > Activities and Due Dates Ch23 /21/2018 12:00 AM 34.4/1004/14/2018 05:50 PM Print CalcuatorPeriodic Table Gradebook stion 4 af 8 Sapling Learning A solvent passes through a chromatography column in 3.5 minutes, but the solute requires 9.5 minutes. (a) What is the retention factor, K? (b) What fraction of the time does the solute spend in the mobile phase in the column? Number Number The volume of the stationary phase is 0.15 times the volume of the mobile phase in the column (c) What is the partition coefficient, K, for this system? Number

Explanation / Answer

a)

retention factor = ( total time - time taken by solvent)/time taken by solvent

                         = 9.5 - 3.5 / 3.5

retention factor = 1.71

b)

Fraction of the time the solute spends in the mobile phase

                      = time spent by solvent/ total time

                      = 3.5 / 9.5

Fraction of the time = 0.368

c)

Partition coefficient = Retention factor x Vm/Vs

                              = 1.714 x Vm / 0.15 Vm

Partition coefficient = 11.4

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