5) Which step of PCR involves heating the DNA to 9 a) Annealing step b) Extensio

Question

5) Which step of PCR involves heating the DNA to 9 a) Annealing step b) Extension step c) Finishing step d) Denaturation step ands? 4°C in order to separate the str 6) a) RNA primers b) DNA primers c) Genes d) Introns specify which part of the DNA will be amplified by PCR. 7) The portion of RNA that is removed during RNA splicing is called the A Extrorn B Gene C Exon D Intron a product without an 8) PCR products containing an ALU insertion will be insertion. a) Shorter than b) Longer than c) The same size as d) Either larger or smaller depending on the person than 9) To extract DNA from your cheek cells we will a) Heat the cells to rupture them b) Mix the cells with InstaGene matrix c) Denature DNAses that degrade DNA d) All of the above

Explanation / Answer

5) d) Denaturation step

PCR involves three major stages:

They are :

1.    Denaturing – when the double-stranded template DNA is heated to separate it into two single strands.

2.    Annealing – when the temperature is lowered to enable the DNA primers to attach to the template DNA.

3.    Extending – when the temperature is raised and the new strand of DNA is made by the Taq polymerase enzyme.

6) b) DNA primers

PCR reaction is an amplification technique for cloning specific or targeted parts of a DNA sequence to generate thousands to millions of copies of DNA of interest..

7)D Intron

When an RNA transcript is first made in a eukaryotic cells, it is considered a pre-mRNA and must be processed into a messenger RNA (mRNA). In splicing, some sections of the RNA transcript (introns) are removed, and the remaining sections (exons) are stuck back together. A

8)b) Longer than

If the intron does not contain the Alu insertion it is only 641 base pair long as opposed to 941 base pairs long if Alu is present. This increase in size is due to the 300 base pair sequence contributed by the Alu insert.

9) d)All the above.

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