Available Fro Sapling Learning Mapd A Due Date A biochemistry laboratory student

Question

Available Fro Sapling Learning Mapd A Due Date A biochemistry laboratory student used the Bradford protein assay to measure the l content in egg whites. The Bradford protein assay is a spectrophotometric techn Coomassie Brilliant Blue, whose absorption undergoes a spectroscopic shift when bound to a protein. In the unbound state, Coomassie Brilliant Blue displays a red color. As protein binds to the dye, its absorbance at 595 nm increases and the dye changes to a blue color. The student prepared a 3.000 mL sample containing 2 mg/mL Coomassie Brilliant Blue. To this sample, 300.0 pL of 75.0 ug/mL lysozyme was added and an absorbance of 0.278 was measured at 595 nm. Calculate the corrected absorbance for this sample. Points Possibl Grade Catega Description Policies: ysozyme (protein) ique that uses a dye, You can chec You can view give up an an You can keep untl you get You lose 5 answer in you attempt at th Number Corrected absorbance 3.058 O eTextbook O Help With Th There is a hint availablel O Web Help & Viow the hint by clicking on the bollom divider bar Click on the divider tar ouain to huli, ?he hint Close O Technical Su

Explanation / Answer

corrected absorbance = (final volume / initial volume) x observed absorbance

final volume = 3.00 + 0.3 = 3.3 mL

initial volume = 3.000 mL

observed absorbance = 0.278

corrected absorbance = (3.3 / 3 ) x 0.278

corrected absorbance = 0.3058

Related Questions

Navigate

• Browse (All)
• Browse A
• Subjects

---

---

Integrity-first tutoring: explanations and feedback only — we do not complete graded work. Learn more.