C. Denaturation of proteins by organic acias Strong (highly lonized) organio aci

Question

C. Denaturation of proteins by organic acias Strong (highly lonized) organio acide sueh as trlchloroacetie acid and pierie acid react with positively charged side chains on proteins to form a precipitate and disrupt proteinstructure a illustrated belows protein backbone native protein denatured procein 1. To a test tube containing 3 mt of 2 BSA, add 1-2 mt 5 trichloroacetic aci4 ribe any changes. C. Questions 1. Explain the changes in terms of bonde and levelo of structure disrupted. 2. Write an equation showing this dieruption. 3. Name 3 amino acids whose side chaine may contribute positively-charged groups.

Explanation / Answer

C1. TCA causes conformational changes in protein that causes its precipitation.

TCA generally effects the secondary structure of BSA through lysozyme.

2. (TCA) = RPo[l - e-(kr+kd)t]

Po = Initial amount of BSA , kr and kd are rate contants for regurgation and degradation respectively.

3. Basic amino acids like lysine, arginine, histidine contribute + charged groups.

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