Determination of the Molar Absorption Coefficient for Nucleosides MIROOTbeebso n

Question

Determination of the Molar Absorption Coefficient for Nucleosides MIROOTbeebso nd a nucleotide? 2. What is the Amax and the absorption coefficient (at the Amax) for each of the following nucleosides (Guanosine, Cytidine, Uridine, Adenosine, Thymidine and Inosine)? Make sure that you cite your literature source for this information. Absorption Coefficient Nucleoside Amax (nm) Reference 3. You have a stock solution of Inosine that when diluted with water 1:20 will give an absorbance at 248.5 nm of approximately 0.85. What was the concentration of the stock solution of Inosine in mol/L?

Explanation / Answer

1.)Nucleosides are glycosylamines consisting of a nucleobase bound to ribose or deoxyribonucleic ribose sugar via a beta-glycosydic linkage. Cytidine , thymidine , guanosine are some examples .

Nucleotide is composed of nucleobase , a nitrogenous Base of five carbon sugars and one two or three phosphate groups forming bonds with 2,3 or 5 th carbon of sugar . 5 carbon site is most common .

Nucleic acid bases are nitrogen containing biological compounds that form nucleoside which in turn are compounds of nucleotide together are monomers constituting basic building block of nucleic acids . Purine and pyramidine bases together forms it .

2.)

Absorption

max

absorption

Coefficient

(M-1cm-1)

uv absorption spectroscopic datas

James McT.Ploeser and Hubert S Loring

3.)By beer lambert law we know that

A=£cl , where £ is molar extinction coefficient , A is absorbance , l is pathlength and c is concentration .

Molar extinction coefficient and pathlength is needed to calculate concentration . Here 248.5nm is just indicating that there is a possible absorption .

By referring data from --Urban M, joubert N, purse n etal mechanism by which human DNA chooses to polymerase a nucleoside triphosphate (2010)biochem (49):727-735-- data path length taken as 1 cm and £=12300Lmol-1cm-1

C=0.85/1*12300*0.01

=0.85*0.01 mol/litre .

Absorption

max

absorption

Coefficient

(M-1cm-1)

References Guanosine 275nm 8400

uv absorption spectroscopic datas

James McT.Ploeser and Hubert S Loring

Cytidine 270nm 8830 " " " Uridine 262nm 9820 " " " Adenosine 259nm 13400 M. Dwarkin and K H Keller Thymidine 267nm 9560 A I Nikoluev and SV Patson 2015solvent type influence on thymidine uv spectroscopy Inosine 249nm 12500 Glen research lab flourosent data

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