2018 Thin-Layer Chromat Pre-Lab Questions: . Use n unkno ture 1. How specificall

Question

2018 Thin-Layer Chromat Pre-Lab Questions: . Use n unkno ture 1. How specifically do these factors influence how far up a polar TLC plate a compound will mi- grate? e polarity of the compound . polarity of the mobile phase which . Salicyl known 2. Why would the following spotted plates fail toe pestle so you next st . Use a give good results by TLC? Lywskva should tom of of mob position . Perform b. 3. Why should the spots of compound on the chromatography plate be as small as possi- ble? plates Post-Lab Questions: center l Reading-Guide-1 docx Read

Explanation / Answer

1. A. The TLC is polar. If compound is also polar then the solute will bind more to mobile phase and migrate less.

B. If mobile phase is more polar then polar solute will bind more to mobile phase and migrate more.

2. A. Fail because the RF value is very less to compare.

B. Fail because the spot donor move at all.

C. Fail because the both solute have same RF value.

D. Fail because the spots joined and No clear spot.

3. They should be small because then would give clear spot and show proper movement. They would not leave trail.

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