ab 3: Separation of the Pigments in Spinach by Thin Layer Chromatography 2. Expl
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Question
ab 3: Separation of the Pigments in Spinach by Thin Layer Chromatography 2. Explain the effect on the Ry values if the surface of the plate is broken while drawing the pencil line or while applying a spot 3. What is the purpose of the pencil line? 4. Explain the effect of not drying a spot before the next addition of pigment. 5. Explain the effect on the Rr factor of applying too large a spot 6. Explain the effect of moving the developing tank while a plate is being developed 7. What would happen if you removed your plate to add another spot?Explanation / Answer
2. If the surface of the plate is broken, the spots would not move smoothly on the plate and therefore Rf value would be inaccurate.
3. Pencil line is made to mark the solute front, the slovent front. Both of which is needed to calculate Rf value.
4. If the spot is not dried before applying the next addition of pigment, the spots would becomes bigger by expansion and bluring (unclear) of separation would be seen.
5. If the spots are too big then bluring of spots on the plate would be seen. It would be difficult to meaure the Rf value from the distance moved for such an spot.
6. Uneven solvent layer would reach the spots If the solvent chamber is moved while the plate is developing, the spots movement will not be even.
7. If the plate is removed in between to add another spot, the first spot would move farther up then the spot applied later on the plate.
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