You receive results from Sanger sequencing of an expression construct. You are s

Question

You receive results from Sanger sequencing of an expression construct. You are sequencing the region containing the start codon that corresponds to the amino- terminus of the protein. You expect to see the sequence 5 -ATGGCCGTACGTGGTACG-3, but the sequence you receive is 5 -ATGGCCGGTACGTGGTACG-3 How will this change in sequence affect the recombinant protein you express? (A) Your construct will produce only a dipeptide. (B) Your construct will not produce the correct sequence after the second amino acid. (C) Your construct will produce the expected protein. (D) Your construct will produce no protein. (E) None of the above are correct.

Explanation / Answer

three bases will make up an amino acid so here the 1st and 2nd amino acids are ATG and GCC

after that one extra base has been inserted.so every codon after that insertion will get changed.

it is considered as a "frameshit mutation "in case of DNA because it shift the reading frame of genetic message.The amino acids choosen will change their functions and the protein may be caused to malfunction

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