Biochemistry What is the principle behind using ammonium molybdate in the determ
ID: 1027046 • Letter: B
Question
BiochemistryWhat is the principle behind using ammonium molybdate in the determination of phosphate in different samples?
(P.s. , please answer in a discussion way, and please provide sources as well. Also, please type it as well, thank you so much!) Biochemistry
What is the principle behind using ammonium molybdate in the determination of phosphate in different samples?
(P.s. , please answer in a discussion way, and please provide sources as well. Also, please type it as well, thank you so much!)
What is the principle behind using ammonium molybdate in the determination of phosphate in different samples?
(P.s. , please answer in a discussion way, and please provide sources as well. Also, please type it as well, thank you so much!)
Explanation / Answer
Phosphate is readily reacted with ammonium molybdate in the presence of some suitable reducing agents and gives a blue colored complex.
Phosphate + ammonium molybdate ===) phosphomolybdates
Phosphomolybdates + Ferrous ammonium sulfate ===) molybdenum blue.
The intensity of blue colored solution is increased with the concentration of the phosphate content in the sample. The blue colored complex absorbance is measured by colorimetric technique. The unknown sample phosphate content value is measured by calibration curve of a series of known concentration of phosphate in the complex form of molybdenum blue.
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