Part of the mature mRNA sequence of a eukaryotic protein reads as follows: GGACC

Question

Part of the mature mRNA sequence of a eukaryotic protein reads as follows: GGACCUCCGGGCCCCCCA. This sequence is presented in-frame (i.e. the first three bases shown make up a codon, the next three make up another codon, and so forth), and this sequence (or slight variations thereof) appears repeatedly in the full-length mRNA. If the full-length mRNA was translated and post-translationally processed to the corresponding mature protein, and if the mature protein was completely hydrolyzed with 1N HCl, what would be the observed molecular weights of the most prevalent amino acids thus obtained? (Hint: use 1 for the atomic weight of hydrogen, 12 for the atomic weight of carbon, 14 for the atomic weight of nitrogen, 16 for the atomic weight of oxygen, and 32 for the atomic weight of sulfur, as needed.)

Explanation / Answer

The given mRNA sequence of a eukaryotic protein is ......GGACCUCCGGGCCCCCCA....... The 3 nucleotides make up a CODON . i.e

Different codons present in this given sequence are :-   GGA  CCU CCG GGC CCC CCA

If this sequence of mRNA was translated then we obtain an amido acid sequence i.e

Gly - Pro - Pro - Gly - Pro - Pro

and when this sequence of amino acid undergoes complete hydrolysis ,the most prevalent amino acid that is obtained is PROLINE ( Pro ) .The molecular formula of proline is C5H9NO2.

Thus ,molecular weight will come out to be 115g.

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