SHUW ALL WORK FOR MATHEMATICAL QUESTIONS! (1) The equation A = abc becomes nonli
ID: 1025174 • Letter: S
Question
SHUW ALL WORK FOR MATHEMATICAL QUESTIONS! (1) The equation A = abc becomes nonlinear at high concentrations of the absorbing substance. Suppose you prepare a solution with a very high absorbance that is suspect in NOT following the linear relationship. How might you still use the sample for your analysis, rather than discarding the sample and the data? (1) Fingerprint smudges are present on the cuvet containing the solution placed into the spectrophotometer for analysis. How does this technique error affect the absorbance reading for FeSCN in the analysis?(Increase, decrease or no effect) (1) EXPLAIN. (1) (3) Glass cuvets, used for precision absorbance measurements in a spectrophotometer, are marked so that they always have the same orientation in the sample compartment. What error does this minimize and why? (1) (4) A 0.0750 M sample of Co(NO3)h gave an absorbance of 0.356 at 505 nm in a 1.00 cm cell. What is the cobalt concentration of a solution giving an absorbance of 0.361 in a 2.0 cm cell ? (2)Explanation / Answer
1)
Since the absorbance is very high, we have to dilutethe solution to a known concentration where the absorbanceis with in the limits.
2)
Fingerprints on a cuvette would affect absorbance and concentration calculation by the fact that fingerprints absorb and scatter light slightly, despite not be readily visible.
A cuvette with fingerprints on it will give a slightly higher absorbance reading and the measured concentration will be inaccurate
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