TnN nF MIITAGENS / CARCINOGENS . AMES TEST e-r 21) Consider the following 5 stra

Question

TnN nF MIITAGENS / CARCINOGENS . AMES TEST e-r 21) Consider the following 5 strains of fungi that are all unable to produce a necessary vitamin molecule X. The biosynthetic pathway associated with the production of X begins with a precursor molecule called U. Please produce the appropriate biosynthetic pathway for the following information. Locate and show the position of the mutant enzymes (labeled A thru E) associated with each of these strains. Locate and show the position of the intermediate molecules (labeled S, T, V, W, Y) associated with this pathway. particular intermediate or final molecule. A plus sign represents growth of the strain when supplemented with the STRAIN MOL. V MOL. x MOL.Y MOL. W MOL.S MOL. T WILD since they are 1. Al of the strains labeled A thru E are referred to as a vitamin unable to produce the vitamin X 2. The wild type strain that is able to produce the vitamin when given just the basic precursor molecules (simple salts, a sugar source and biotin) should be referred to as a- --. 3. Label the appropriate pathway components for the strains and their associated enzyme mutations as well as the molecules. Mutant Mutant Mutant Mutant 4. From the information given above it is dlear that all of the mutant strains must be able to convert the precursor molecule (U) to the second molecule in the pathway. (True or False). 5. The individuals that were responsible for first suggesting the one gene one enzyme hypothesis utilizing Neurospora mutants were and

Explanation / Answer

1) vitamin x auxotrophs- auxotrophs are the organisms that are unable to produce a specific compound which is required for their growth and metabolism.

2)Prototrophic strain: prototrophic strains have the ability to synthesize all the compounds needed for growth.

3)

4) True: all the strains are able to convert precursor molecule (U) to secondary molecule X in the pathway.hece they are shown as +.

5)George Beadle and Edward Tatum.

mutant D mutant A mutant c mutant E mutant B mole x mole v mole t mole w mole s

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