2) a. Each oligonucleotide listed below is used to prime a separate in vitro tra

Question

2)
a. Each oligonucleotide listed below is used to prime a separate in vitro translation system. Remember this is an artificial system, initiation is random, limit reactions to only tripeptides:

1. AGAGAGAGAG

2. GAGGAGGAGGAG

3. CAGACAGACAGA

b. The peptide products from each reaction are isolated, sequenced and listed below.

1. arg-glu-arg, and glu-arg-glu

2. gly-gly-gly, glu-glu-glu, and arg-arg-arg

3. asp-arg-gln, gln-thr-asp, thr-asp-arg, and arg-gln-thr

c. Without using a genetic code table, explain how one assigns an amino acid to the potential codons in the listed oligonucleotides.

Explanation / Answer

Each amino acid is paired up with a three base codon. such as arg is paired with AGA, so everytime you see an AGA you know there is a arg that will match with it. So the first one works as arg-glu-arg if you start from the very first A and continue down the strand so there is a left over G, It also works as a glu-arg-glu if you skip the first A in the sequence and then continue to the end. For part two you would pair gly with GAG, glu with GGA and Arg with AGG, you are able to pair arg with AGG thus producing all three products. I will leave part three for you too figure out on your own just pick a sequence of three bases to represent each amino acid and move where you start along the chain for each new peptide product

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