Given the table of data below for the reaction of o-diphenol oxidase with catech

Question

Given the table of data below for the reaction of o-diphenol oxidase with catechol, draw the Lineweaver-Burk plot and find the slope of the graph and use the value of the y-intercept (1/V_max)to obtain the K_M value. Compare that value to the reciprocal of the x-intercept where (1/V) = 0. Submit your graph along with your calculations. We will need three significant figures to compare with the data in the next problem to see the effect of an inhibitor. The values for (1/V_i) have been rounded to three significant figures for a smoother lit to the V_i values, which are only given to two significant figures. However, Prof. Kimball rounds (1/V_1) further to only two significant figures. In either event, biological data are often more scattered than physical data but some of the (1/S) data are rounded to three significant figures; so we carry three significant figures in (1/V_1). When using biological data, it is essential to use least-squares fits.

Explanation / Answer

Lineweaver-burk plot is

1/V= (KM/Vmax)1/S +1/Vmax

the plot of 1/V vs 1/S gives The intercpet if 1/Vmax and slope is KM/Vmax

the plot is shown below

KM/Vmax= 11.91 and 1/Vmax= 9.926 Vmax = 1/9.926=0.100746

KM= 11.91*Vmax= 11.91*0.100746=1.199

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