It is suspected that the gene DEFT regulates the expression of many other genes.
ID: 101777 • Letter: I
Question
It is suspected that the gene DEFT regulates the expression of many other genes. How could one test this?
Use CRISPR to knock DEFT out, then use RNAi to examine how genes are expressed with and without DEFT expression.
Use CRISPR to knock DEFT out, then use microarrays to examine how genes are expressed with and without DEFT expression.
Use microarrays to knock out DEFT, then use RT-PCR to see how genes are expressed with and without DEFT expression.
Knock out different genes using CRISPR, then see how DEFT is expressed using RNAi.
Knock out different genes using CRISPR, then see how DEFT is expressed using microarrays.
Use CRISPR to knock DEFT out, then use RNAi to examine how genes are expressed with and without DEFT expression.
Explanation / Answer
Use CRISPR to knock DEFT out, then use microarrays to examine how genes are expressed with and without DEFT expression.
CRISPR is short palindromic sequence used mainly to deform bacterial genome and DEFT gene helps in defense mechanism using CRIPSER it is easy to target DEFT gene once it is affected the gene regulation of DEFT gene and gene expression of other genes can be assessed by using microarrays. Microarrays can be used to test the gene expression of genes various genes simultaneously.
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