https://youtu.be/K3-BMFHrsVg On a blank white sheet of paper, sketch a dichotomy
ID: 101482 • Letter: H
Question
https://youtu.be/K3-BMFHrsVg
On a blank white sheet of paper, sketch a dichotomy chart as a flow chart walking through each of your experiments. The experiments were colony morphology, Gram stain, catalase test, MSA plates, penicillin susceptibility, and sheep blood agar plates. Scan or take a picture, and upload this to the Project Drawing Submission folder.
Orange track
Write out the discussion section and references (for this section) for these experiments. Write this as if it were part of the whole lab report in manuscript form. This should be at least three paragraphs and contain at least one additional citation/reference beyond the textbook and lab book for this course. Suggestions for the paragraphs:
1) State each of the results and the interpretation of each experiment.
2) Discuss how all the results fit together here or the first paragraph. Make a conclusion, and state why this is your conclusion (can be repetitive with your experimental results/interpretations). Based on your experimental results, how scientifically confident are you for the genus ID? Species ID?
3) Discuss any results/interpretations that are exceptionally clear and unclear/indeterminate. Are there any alternative conclusions that could be made? Discuss at least one other species that could be a conclusion, and why/why not this is a possibility. State at least one other additional test that could have been done in this series, and additionally how nucleic acid testing (genetic tests) OR serology could confirm the final ID.
Explanation / Answer
The catalase test is used in the given experiment to differentiate between staphylococcus and streptococcus bacterial strains.
Catalase Test: Catalase is an enzyme to convert hydrogen peroxide to water and oxygen gas. The presence of catalase enzyme in the test isolate is detected using hydrogen peroxide. If the bacteria possess catalase (i.e., are catalase-positive), when a small amount of bacterial isolate is added to hydrogen peroxide, bubbles of oxygen are observed.
The coagulase test differentiates Staphylococcus aureus from other staphylococcal species.
Coagulase Test: Coagulase is a protein enzyme produced by several microorganisms that enables the conversion of fibrinogen to fibrin. It is produced by S. aureus that converts (soluble) fibrinogen in plasma to (insoluble) fibrin
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