Why is it helpful to include both positive and negative controls in an experimen
ID: 101154 • Letter: W
Question
Why is it helpful to include both positive and negative controls in an experiment? Imagine you test a material using Lugol's reagent. If this test is negative what can you say about the molecules present in the material? Amylase, lipase, and protease/pepsin are enzymes. Write a complete definition of an enzyme. Use your observations and conclusion from this experiment to write a brief definition of enzyme specificity. Explain why homeostasis is important to enzyme function. Based on the results of your experiment with the egg white (protein), what are the conditions needed for optimal pepsin function? What do the results of your lactase experiment tell you about the presence of different kinds of sugars in almond milk, dairy milk, and lactose-free dairy milk?Explanation / Answer
ANSWER:
1) These two controls, when both are fruitful, are generally adequate to take out most potential bewildering factors: it implies that the investigation delivers a negative outcome when a negative outcome is normal, and a positive outcome when a positive outcome is normal.
2) The molecules appear in the Lugol's reagent if test is negative is yellow-orange.
3) Chemicals are proteins that go about as impetuses inside living cells. Impetuses increment the rate at which concoction responses happen without being expended or for all time changed themselves. A compound response is a procedure that believers at least one substances (known as reagents, reactants, or substrates) to another sort of substance (the item). As an impetus, a protein can encourage a similar compound response again and again.
4) Specificity of Enzymes
One of the properties of proteins that makes them so critical as demonstrative and research devices is the specificity they display with respect to the responses they catalyze. A couple of catalysts display outright specificity; that is, they will catalyze just a single specific response. Different proteins will be particular for a specific kind of concoction bond or utilitarian gathering. By and large, there are four particular sorts of specificity:
Absolute specificity - the compound will catalyze just a single response.
Group specificity - the compound will act just on particles that have particular practical gatherings, for example, amino, phosphate and methyl gatherings.
Linkage specificity - the protein will follow up on a specific sort of substance bond paying little respect to whatever remains of the sub-atomic structure.
Stereochemical specificity - the chemical will follow up on a specific steric or optical isomer.
5) Cells rely upon the body condition to live and work. Homeostasis monitors the body condition and keeps the conditions appropriate for cells to live and work. Without the correct body conditions, certain procedures (eg osmosis) and proteins (eg catalysts) won't work appropriately.
6) Catalyst has an ideal pH range and anything outside this range represses its action , thusly it was estimated that the lower the pH, the fasterthe rate of catalyst response (pepsin) action. Pepsin has an ideal scope of pH 2 , which is profoundly acidic and is found in the stomach of the human bodywhere it digests proteins. This implies any pH less acidic or more essential than thislevel won't enable the response to happen, as these are not the ideal conditions for theactivity of the protein.
7) The cow milk, goat milk, and soy milk all should have shown a negative reading for glucose, even though glucose was present in all three milks. This is because the glucose was bonded with galactose in both the cow’s milk and goat’s milk and with fructose in the soy milk. When the monosaccharides bond to form disaccharides they are no longer present in their individual states. This can be compared to milk that has chocolate powder mixed into it. The powder is no longer present in powder form and the milk is no longer white milk. However, they cannot be separated as lactose can.
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